Microbiological Testing of Metalworking Fluids

Protect Your Equipment, Fluids, and Workforce with Reliable Microbial Testing

Metalworking fluids (MWFs) are necessary for machining operations. They provide lubrication, cooling, and corrosion protection. However, these fluids are vulnerable to microbial contamination. This can affect fluid performance, harm equipment, threaten worker health, and raise operating costs.

Our laboratory offers in-depth testing of metalworking fluids for microbial contamination. This includes bacteria, fungi, and mycobacteria, as well as challenge testing to assess how well biocides and fluid formulations work.

Why Test Metalworking Fluids for Microorganisms?

1. Ensure Fluid Performance

Microbial growth in MWFs degrades fluid components, leading to poor lubrication, foul odors, and shortened fluid life. Regular testing helps detect early contamination, allowing for timely treatment and maintenance.

2. Protect Worker Health

Exposure to microbially contaminated fluids, especially those containing Mycobacterium immunogenum and other opportunistic pathogens, has been linked to respiratory illnesses such as hypersensitivity pneumonitis, also known as “machine operator’s lung”. Monitoring metalworking fluids helps reduce health risks for workers.

3. Prevent Equipment Damage

Microbes can cause fluid instability, emulsification issues, and microbial-induced corrosion (MIC), leading to costly downtime and equipment repairs.

Routine Testing of Used Metalworking Fluids

We analyze in-use metalworking fluid samples for microbial load using standardized culturing methods. Testing can be tailored to your specific needs and typically includes:

  • Total Aerobic Bacteria Count (CFU/mL)
    Detects the general bacterial population, including species that degrade emulsifiers and additives.
  • Fungal (Yeast and Mold) Count (CFU/mL)
    Detects fungal species that may form biofilms and slime, causing filter clogging and corrosion.
  • Mycobacteria Detection
    Specialized testing using selective media to detect Mycobacterium immunogenum, a known health risk in MWF systems.

Challenge Testing of Metalworking Fluids

In addition to in-use fluid testing, we provide controlled challenge testing in our laboratory to evaluate the antimicrobial performance of metalworking fluid formulations and biocides.

What is Challenge Testing?

Challenge testing introduces known concentrations of microbial species into metalworking fluid samples under controlled conditions to assess how well the fluid or additive inhibits growth.

Microbes Used in Challenge Testing:

  • Gram-negative bacteria such as Pseudomonas aeruginosa
  • Fungi such Aspergillus niger
  • Mycobacteria, especially Mycobacterium immunogenum

Contact us today to schedule your testing or request a quote.

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